Wayne Bridge pulled out of training yesterday and will miss his chance to play at left-back.
With Ashley Cole already sidelined, this was a chance for Bridge to prove his worth at club and international level with a run in both sides.
England and Blues skipper John Terry will also not play. JT trained with the squad, but has said he is not ready and will take it easy ahead of his club side's match on Saturday against Middlesbrough.
Shaun Wright-Phillips, who was an impressive sub againsr Charlton on Saturday, will be on the bench against the Spanish.
Chelsea have a host of players on international duty this week, and Jose Mourinho must be crossing his fingers that his stars come back unscathed.