The Chelsea midfielder suffered a thigh injury in training on Wednesday and went for a scan on Thursday afternoon to discover the extent of the damage. However, Lampard and manager Roy Hodgson were preparing for the worst and the Football Association have now confirmed the player will not be part of England's 23-man squad.
Jordan Henderson will be called into the England squad providing Hodgson gets the green light from UEFA, which should be automatic as Lampard has suffered a fresh injury after Tuesday's deadline.
It is devastating news for England, who have already had to deal with the loss of one midfielder, Manchester City's Gareth Barry who suffered a stomach injury in Saturday's friendly win over Norway.
And for Lampard, it potentially spells the end of his entire England career, with Hodgson expected to offer the younger midfielders a chance to prove themselves during next autumn's World Cup qualifying programme.
What makes this latest twist even more galling is that Lampard was only in his second day of training, having joined the Three Lions party late after winning the Champions League with his club in Munich on May 19.
Hodgson said: "It's a huge blow for the team, especially coming after the loss of another senior player in Gareth Barry.
"The fact that the doctor cannot guarantee Frank could take part in any of the group matches means we have to replace him in the central midfield area. Apart from it being a huge blow to me and the team, I'm especially disappointed for Frank himself.
"He has been in very good form and was looking forward to the tournament.
"He made a huge contribution towards us qualifying for the Euros so it is cruel to be forced out through injury just before the tournament."
Source: PA
Source: PA